Search results for "metabolism [Insulins]"

showing 3 items of 3 documents

Multi-approach metabolomics analysis and artificial simplified phytocomplexes reveal cultivar-dependent synergy between polyphenols and ascorbic acid…

2017

Fruits of the sweet cherry (Prunus avium L.) accumulate a range of antioxidants that can help to prevent cardiovascular disease, inflammation and cancer. We tested the in vitro antioxidant activity of 18 sweet cherry cultivars collected from 12 farms in the protected geographical indication region of Marostica (Vicenza, Italy) during two growing seasons. Multiple targeted and untargeted metabolomics approaches (NMR, LC-MS, HPLC-DAD, HPLC-UV) as well as artificial simplified phytocomplexes representing the cultivars Sandra Tardiva, Sandra and Grace Star were then used to determine whether the total antioxidant activity reflected the additive effects of each compound or resulted from synergis…

0301 basic medicineantioxidantAntioxidantmedicine.medical_treatmentOrganic chemistrylcsh:MedicineAscorbic AcidBiochemistry01 natural sciencesAntioxidantsMass SpectrometryAnalytical ChemistryPrunusSpectrum Analysis Techniquesartificial phytocomplexMetabolitesVitamin CPrunus avium L.Cultivarlcsh:ScienceCherriesChromatography High Pressure LiquidLiquid ChromatographyMicroscopyMultidisciplinaryChromatographic TechniquesLight Microscopyfood and beveragesVitaminsPlantsPhysical sciencesChemistryHorticultureItalyMetabolomesecondaryResearch ArticlePrunus avium L. antioxidant secondary metabolism synergy artificial phytocomplexmetabolism synergyFluorescence Recovery after PhotobleachingLiquid Chromatography-Mass SpectrometryPrunus aviumBiologyResearch and Analysis MethodsFruitsChemical compounds03 medical and health sciencesMetabolomicsSpecies SpecificityOrganic compoundsBotanymedicineMetabolomicsGenetic variabilityNuclear Magnetic Resonance Biomolecular030109 nutrition & dieteticsVitamin C010401 analytical chemistrylcsh:ROrganismsBiology and Life SciencesPolyphenolsAscorbic acid0104 chemical sciencesMetabolismPolyphenolFruitMultiprotein ComplexesLinear Modelslcsh:QPLoS ONE
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Functional Metabolic Diversity of Bacterioplankton in Maritime Antarctic Lakes

2021

A summer survey was conducted on the bacterioplankton communities of seven lakes from Byers Peninsula (Maritime Antarctica), differing in trophic and morphological characteristics. Predictions of the metabolic capabilities of these communities were performed with FAPROTAX using 16S rRNA sequencing data. The versatility for metabolizing carbon sources was also assessed in three of the lakes using Biolog Ecoplates. Relevant differences among lakes and within lake depths were observed. A total of 23 metabolic activities associated to the main biogeochemical cycles were foreseen, namely, carbon (11), nitrogen (4), sulfur (5), iron (2), and hydrogen (1). The aerobic metabolisms dominated, althou…

Microbiology (medical)maritime Antarctic lakesBiogeochemical cyclemicrobial co-occurrence networkQH301-705.5FAPROTAXGrowing seasonMicrobiologyArticleNutrientVirologyparasitic diseasesOrganic matterBiology (General)metabolism inferenceByers PeninsulaTrophic levelchemistry.chemical_classificationBiomass (ecology)Biolog EcoplatesEcologyBacterioplanktonbiogeochemical cyclesfunctional diversitychemistryEnvironmental sciencenext-generation sequencingEutrophicationMicroorganisms
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Heat shock protein 27 is involved in SUMO-2/3 modification of heat shock factor 1 and thereby modulates the transcription factor activity

2009

Heat shock protein 27 (HSP27) accumulates in stressed cells and helps them to survive adverse conditions. We have already shown that HSP27 has a function in the ubiquitination process that is modulated by its oligomerization/phosphorylation status. Here, we show that HSP27 is also involved in protein sumoylation, a ubiquitination-related process. HSP27 increases the number of cell proteins modified by small ubiquitin-like modifier (SUMO)-2/3 but this effect shows some selectivity as it neither affects all proteins nor concerns SUMO-1. Moreover, no such alteration in SUMO-2/3 conjugation is achievable by another HSP, such as HSP70. Heat shock factor 1 (HSF1), a transcription factor responsib…

Protein sumoylationTranscriptional ActivationCancer Researchendocrine systemanimal structuresSUMO proteinHSP27 Heat-Shock ProteinsBiologyurologic and male genital diseasesenvironment and public healthSubstrate Specificity03 medical and health sciencesTransactivation0302 clinical medicineHeat Shock Transcription FactorsHeat shock proteinGeneticsAnimalsHumansAnimals Cell Nucleus/metabolism DNA-Binding Proteins/*metabolism HSP27 Heat-Shock Proteins/chemistry/*metabolism Hela Cells Humans Protein Multimerization Protein Structure[SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular BiologyHSF1Protein Structure QuaternaryMolecular BiologyTranscription factorUbiquitinsHeat-Shock Proteins030304 developmental biologyCell Nucleus0303 health sciencesMolecular biologyHsp70Cell biologyHeat shock factorDNA-Binding ProteinsProtein TransportQuaternary Protein Transport Small Ubiquitin-Related Modifier Proteins/*metabolism Substrate Specificity Transcription Factors/*metabolism Transcriptional Activation Ubiquitins/*metabolism030220 oncology & carcinogenesisembryonic structuresSmall Ubiquitin-Related Modifier ProteinsProtein MultimerizationHeLa CellsMolecular ChaperonesTranscription Factors
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